Was ist SS-31?
SS-31 (elamipretide) is a small, aromatic peptide that easily penetrates cell and organelle membranes.It is thought to interfere with the production of reactive oxygen species (ROS or free radicals) and promote energy production in cells by stabilizing the enzyme cardiolipin within mitochondria. Cardiolipin is part of the inner mitochondrial membrane where it acts as a fundamental component of the electron transport chain, the machinery by which most energy need for cellular functioning is produced.
Dysfunction of cardiolipin has been implicated as contributing to the pathology of a number of diseases including Alzheimer’s disease, Parkinson’s disease, nonalcoholic fatty liver disease, diabetes, heart failure, HIV, cancer, chronic fatigue syndrome, and more. Cardiolipin is thought to be a major component of mitochondrial myopathy, which isn’t a single disease but rather is a group of neuromuscular disorders caused by damage to mitochondria. Mitochondrial myopathy is characterized by everything from muscle weakness and exercise intolerance to heart failure, seizures, and dementia. SS-31 is the first peptide to ever undergo clinical trials as a potential treatment for mitochondrial myopathy.
Verbesserung der Mitochondrien
Primary mitochondrial diseases (PMDs) are among the most common inherited conditions in the world. They are caused by dysfunction in the energy-producing apparatus of mitochondria. Symptoms vary greatly between forms of the disease, but the most susceptible organ systems are those with high energy demands (e.g. nervous system, heart, kidney, etc.). Muscle involvement and exercise intolerance are almost universal in mitochondrial disorders. Common symptoms include easy fatigue, exercise intolerance, and seizures.
PMDs, and mitochondrial diseases in general, are primarily characterized by disturbances in the production of ATP. ATP acts as the energy currency of the cell and is necessary to almost every cell function. Stabilizing ATP production in the setting of mitochondrial disease has long been a goal of the medical profession. With the development of SS-31, that goal may final have been realized.
The first evidence that SS-31 could restore energy production in PMDs came from animal studies. In that research, rats who had suffered ischemia-perfusion injury (a non-genetic cause of mitochondrial disease) of the kidney were given SS-31. The peptide protected kidney structure, accelerated recovery of ATP production, and reduced cell death and necrosis within the kidney[1]. Subsequent studies in mice showed that SS-31 interacted with cardiolipin in the inner mitochondria membrane and revealed that the peptide could reduce symptoms of mitochondrial disease regardless of etiology. There is also evidence that it can improve mitochondrial dysfunction that results from age[2]–[4]. From these findings, it was relatively simple to convince the FDA to grant orphan drug status to SS-31 and pave the way to clinical trials.
In phase II trials in humans, SS-31 increased exercise performance after just 5 days of treatment and showed no safety concerns or prominent side effects[5]. Unfortunately, phase III trials failed to produce convincing evidence of SS-31’s clinical utility[6]. That said, there is good reason to believe that the trial endpoints simply weren’t appropriate and that additional work will result in the peptide being approved for the treatment of certain mitochondrial conditions. According to Dr. Bruce Cohen, director of the Neurodevelopment Science Center at Akron Children’s Hospital, the results for prior phase II clinical trials were very encouraging and so it is not time to give up. Rather, he notes, SS-31 should spur interest in this particular area and bring other big pharma research to the table[7]. It appears that that is already happening as the company that first brought SS-31 to clinical trials is planning to move forward with trials of a derivative of SS-31 as well as trials investigating other endpoints for SS-31 treatment[6].
As of right now, SS-31 is being tested in a number of different human diseases and under a number of different trial models. The peptide is considered safe to use in humans, so it can also be prescribed by doctors under compassionate care exceptions to patients who have no other treatment options. The peptide will likely become part of mainstream medical care for a number of conditions in the near future, but even now it is available to people who need it while clinical trial work is ongoing.Ischämie
Perhaps the most compelling secondary application of SS-31 is in the treatment of heart failure. It has long been known that heart failure causes negative changes to the function of mitochondria and that these changes, in a kind of destructive cycle, cause heart failure to worsen. Research in human heart tissue treated with SS-31 shows significant improvements in mitochondrial oxygen flux and activity of specific components involved in the production of ATP. This particular study was carried out in a manner that precluded cardiolipin restructuring, however, suggesting that SS-31 may have a second mechanism of action on mitochondrial function that needs to be explored[4]. This finding has actually been replicated in a number of research studies, strengthening the idea that SS-31 is not just useful for restoring ATP production via cardiolipin interaction. The peptide is actively being investigated for its ability to alter the production of reactive oxygen species and improve mitochondrial function in both acute and chronic use situations.
Studies in dogs, for instance, show that chronic treatment with SS-31 can improve left ventricular function in the setting of advanced heart failure. Measures of mitochondrial respiration and maximum ATP synthesis correlated well in this study with overall improvement in left ventricular function implying that SS-31 could be an effective long-term treatment for improving energy dynamics and reducing cardiac remodeling in advanced heart failure[8].
Trials exploring the use of SS-31 in ST-segment elevation myocardial infarction (heart attack) found that the peptide can drastically reduce levels of HtrA2. HtrA2 is a measure of cardiomyocyte apoptosis. These results suggest that SS-31 may be useful in the context of acute heart attack to reduce the extent of injury and preserve cardiac tissue[9].
One role of mitochondrial-targeted therapy in heart failure:
Quelle:PubChem
Diabetes
Diabetes ist zwar scheinbar durch eine einfache Unzulänglichkeit in der Insulinsekretion oder -funktion verursacht, aber eine komplexe Erkrankung mit mehreren pathophysiologischen Manifestationen. In den letzten Jahren wurde das Interesse an der Rolle der mitochondrialen Beeinträchtigung bei der Pathogenese der Krankheit, insbesondere bei Typ -2 -Diabetes, ein gewachsenes Interesse geweckt. Die Behandlung mitochondrialer Dysfunktion wäre daher ein Weg, um einige der langfristigen Folgen von Diabetes wie oxidativen Schäden an kleinen Gefäßen zu verbessern. In einer Studie am Menschen mit SS-31 wurde eine deutliche Abnahme der Produktion von reaktiven Sauerstoffspezies beobachtet. Dies deutet darauf hin, dass SS-31 dazu beitragen kann, die oxidativen Schäden zu verringern, die normalerweise mitochondriale Dysfunktionen begleiten und daher das Fortschreiten der mikrovaskulären Erkrankung bei Typ-2-Diabetes verlangsamen oder stoppen können. Diese Hypothese wird durch den Befund in derselben Studie weiter bestätigt, dass SS-31 die SIRT1-Spiegel erhöhte. Die SIRT1 -Spiegel wurden mit einer verbesserten Insulinsensitivität und einer verringerten Entzündung bei Typ -2 -Diabetes in Verbindung gebracht [10].Reduziert Entzündungen
A theme throughout the sections above is inflammation and the ability of SS-31 to reduce it. In particular, SS-31 appears to be a potent regulator of reactive oxygen species (free radicals) and thus helps to reduce the serious oxidative stress that arises from long-term illness such as diabetes, heart disease, and more. Research in cell cultures suggests that SS-31 reduces inflammation and oxidative stress by reducing expression of FIS1[11]. FIS1 is a mitochondrial protein that is important for mitochondrial growth and division. Elevated levels of FIS1 have been observed in a number of neurodegenerative diseases as well as a variety of cancers and are thought to be evidence of dysfunctional mitochondrial division secondary to dysfunction and inflammation.
There is also good evidence from mouse models to show that SS-31 reduces levels of the inflammatory cytokine CD-36, reduces expression of activated MnSOD, suppresses NADPH oxidase function, and inhibits NF-kappaB p65[12]. All of these are markers of high oxidative stress, so reducing their levels is indicative of reduced free radical production and an improved inflammatory status in the cell. NF-kappaB expression, in particular, is heavily associated with cellular inflammation and is chronically active in a number of inflammatory diseases like rheumatoid arthritis and inflammatory bowel disease. With SS-31, mitochondria do not undergo inflammasome activation, which is to say they don’t convert from the primary production of ATP to primarily producing ROS.
Inflammasome activation is avoided and normal mitochondrial function is preserved in the setting of SS-31 administration:
Quelle:ResearchGate
SS-31-Zusammenfassung
Obwohl SS-31 ursprünglich von Interesse war, weil es angenommen wird, dass die Mitochondrienfunktion bei der Einstellung einer Mitochondrienerkrankung reguliert, gibt es auch gute Hinweise darauf, dass das Peptid mitochondriensinduzierte Entzündungen regulieren kann. Es besteht ein großes aktives Interesse daran, SS-31 zur Verbesserung der Mitochondrienfunktion und damit die allgemeine Energieerzeugung über die ATP-Synthese zu verbessern. Obwohl die anfänglichen Phase -III -Studien nicht erfolgreich waren, wird angenommen, dass dies eher ein Ergebnis der gemessenen Endpunkte im Gegensatz zu einem echten Versagen des Peptids sein könnte, irgendeine Wirkung zu haben. Derzeit gibt es laufende Phase-II-Studien und geplante Phase-III-Studien zum Testen von SS-31 in verschiedenen Krankheitszuständen und mit einer Vielzahl verschiedener Ergebnismaßnahmen. SS-31 kann sehr gut den Schlüssel zum Verständnis der mitochondrialen Dysfunktion bei einer Vielzahl von Krankheiten bieten und sich daher als nützlich für die Gestaltung fortgeschrittener Behandlungen für Alzheimer-Krankheit, Parkinson-Krankheit, Herzerkrankungen, Diabetes, Nierenerkrankungen und mehr erweisen.Artikelautor
Die obige Literatur wurde von Dr. E. Logan, M. D. Dr. E. Logan, untersucht, bearbeitet und organisiertMedizinische Fakultät der Case Western Reserve Universityund ein B.S. in der molekularen Biologie.Autor wissenschaftlicher Zeitschriften
In seiner Arbeit, Behandlungen für Patienten mit mitochondrialen Erkrankungen aufzudecken, ist Dr. Cohen einer der weltweit führenden Experten geworden. Obwohl es keine Heilmittel für Mitochondrienkrankheiten gibt, sucht Dr. Cohen jeden Tag nach einem. Aufgrund seiner Arbeit und des Engagements unseres Krankenhauses, ihm zu helfen, eine Infrastruktur für die Durchführung von klinischen Studien mit mitochondrialen, ist Akron Children eine der führenden Institutionen des Landes, die Behandlungen untersuchten. Bevor Sie sich Akron Childrens bei der Cleveland -Klinik der Cleveland -Klinik anschließen. Er erhielt seinen medizinischen Abschluss am Albert Einstein College of Medicine und absolvierte seine Kinderresidenz im Kinderkrankenhaus in Philadelphia, im Columbia Presbyterian Medical Center und seiner Neuro-Oncology-Stipendium der American Cancer Society in Philadelphia.Bruce H. Cohen, M.D.wird als einer der führenden Wissenschaftler bezeichnet, die an der Forschung und Entwicklung von SS-31 beteiligt sind. In keiner Weise ist dieser Arzt/Wissenschaftler aus irgendeinem Grund den Kauf, Verkauf oder die Verwendung dieses Produkts, der den Kauf, den Verkauf oder die Verwendung dieses Produkts befürwortet. Es gibt keine Zugehörigkeit oder Beziehung, impliziert oder auf andere Weise dazwischen
Peptid-Gurusund dieser Arzt. Der Zweck des Arztes besteht darin, die erschöpfenden Forschungs- und Entwicklungsbemühungen der Wissenschaftler, die dieses Peptid untersuchen, anerkennen, anzuerkennen und zu verdanken. Dr.cohen ist in [5] unter den referenzierten Zitaten aufgeführt.
